Rubicon Antibody

Rubicon (RUN domain Beclin-1-interacting and cysteine-rich-containing protein) is a negative regulator of autophagy, a cellular degradation pathway essential for maintaining neuronal homeostasis. As neurons are particularly vulnerable to the accumulation of damaged proteins and organelles, dysregulation of autophagy is a hallmark of many neurodegenerative diseases, including Alzheimer’s, Parkinson’s, and ALS. Rubicon inhibits autophagosome maturation by interacting with components of the PI3K complex and RAB7A, thereby blocking lysosomal fusion. In the aging brain, elevated Rubicon levels have been associated with impaired autophagic flux and increased vulnerability to proteotoxic stress 1. Notably, Rubicon expression is upregulated in Alzheimer’s disease, where it contributes to amyloid precursor protein (APP) and amyloid-β (Aβ) accumulation. Neuronal overexpression of Rubicon suppresses Aβ clearance, while its knockdown enhances autophagic degradation and reduces extracellular Aβ deposition (1) . Recent findings also highlight Rubicon’s role in mitophagy regulation. Phosphorylation of RAB7A at Ser72 disrupts its interaction with Rubicon, relieving autophagy inhibition and promoting mitochondrial quality control 2. This phospho-switch mechanism underscores Rubicon’s dynamic regulation in response to cellular stress. Given its central role in autophagy suppression and protein aggregation, Rubicon is a promising therapeutic target. Strategies aimed at modulating Rubicon expression or disrupting its inhibitory interactions may restore autophagic balance and mitigate neurodegenerative progression.

Rabbit Anti-Human Rubicon Polyclonal

9007-83-4

Detects ~95 kDa.

Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, KIAA0226, Rubicon, Baron, RUBCN, Beclin-1 associated RUN domain containing protein

12352203

Non-hazardous

Non-hazardous

9711

Q92622

NP_001139114.1

Late Endosome | Lysosome | Early Endosome

Synthetic peptide from the N-terminal of Human Rubicon

Rubicon

Unconjugated

Polyclonal

WB | ICC/IF | IHC

Cardiovascular System | Heart | Cancer | Autophagy | Cell Signaling | Metabolism | Metabolism processes

Peptide Affinity Purified

A 1:1000 dilution of SPC-668 was sufficient for detection of Rubicon in 15 µg of human Hela cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

1 mg/ml

WB (1:1000), ICC/IF (1:100) ; IHC (1:50) ; optimal dilutions for assays should be determined by the user.

0.1

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Blue Ice or 4ºC

-20ºC

Detects ~95 kDa.

Human

Late Endosome | Lysosome | Early Endosome