20(S)-Hydroxycholesterol

20(S)-Hydroxycholesterol

• Alternative Name: 20α-Hydroxycholesterol
• CAS Number: 516-72-3
• UNSPSC Description: 20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells[1][2].
• Target Antigen: Endogenous Metabolite; Smo
• Type: Natural Products
• Related Pathways: Metabolic Enzyme/Protease;Stem Cell/Wnt
• Field of Research: Cancer
• Assay Protocol: https://www.medchemexpress.com/20-s-hydroxycholesterol.html
• Purity: 99.29
• Solubility: DMSO : 12.5 mg/mL (ultrasonic)
• Smiles: C[C@@]1([C@@]2([H])[C@@](C)(O)CCCC(C)C)[C@](CC2)([H])[C@@](CC=C3[C@@]4(CC[C@H](O)C3)C)([H])[C@]4([H])CC1
• Molecular Weight: 402.65
• References & Citations: [1]Nachtergaele S, et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol. 2012 Jan 8;8(2):211-20.|[2]Nedelcu D, et al, Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling. Nat Chem Biol. 2013 Sep;9(9):557-64.
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, 3 years; 4°C, 2 years (Powder)
• Clinical Information: No Development Reported