20(S)-Hydroxycholesterol

• Product Name Alternative:

  20α-Hydroxycholesterol

• UNSPSC Description:

  20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells[1][2].

• Target Antigen:

  Endogenous Metabolite; Smo

• Type:

  Natural Products

• Related Pathways:

  Metabolic Enzyme/Protease;Stem Cell/Wnt

• Field of Research:

  Cancer

• Assay Protocol:

  https://www.medchemexpress.com/20-s-hydroxycholesterol.html

• Purity:

  99.29

• Solubility:

  DMSO : 12.5 mg/mL (ultrasonic)

• Smiles:

  C[C@@]1([C@@]2([H])[C@@](C)(O)CCCC(C)C)[C@](CC2)([H])[C@@](CC=C3[C@@]4(CC[C@H](O)C3)C)([H])[C@]4([H])CC1

• Molecular Weight:

  402.65

• References & Citations:

  [1]Nachtergaele S, et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol. 2012 Jan 8;8(2):211-20.|[2]Nedelcu D, et al, Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling. Nat Chem Biol. 2013 Sep;9(9):557-64.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  -20°C, 3 years; 4°C, 2 years (Powder)

• Clinical Information:

  No Development Reported

• CAS Number:

  516-72-3