RBL1 Antibody

• Background:

  The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  5933

• Swiss Prot:

  P28749

• Accession Number:

  P28749

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RBL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1007-1035 amino acids from the C-terminal region of human RBL1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  121 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RBL1

• NCBI Official Name:

  Retinoblastoma-like protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jayadeva, G., et al. J. Biol. Chem. 285 (39) :29863-29873 (2010)

• Background Reference 02:

  Liu, C.Y., et al. Carcinogenesis 31 (7) :1259-1263 (2010)

• Background Reference 03:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 04:

  Yerges, L.M., et al. J. Bone Miner. Res. 24 (12) :2039-2049 (2009)

• Other Product Names:

  Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, p107, pRb1, RBL1

• Tested Applications:

  WB

• Protein ID:

  90103515

• Physical Properties:

  Liquid