PABPC4 Antibody

• Background:

  Poly (A) -binding proteins (PABPs) bind to the poly (A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  8761

• Swiss Prot:

  Q13310

• Accession Number:

  Q13310

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PABPC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-247 amino acids from the Central region of human PABPC4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  71 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PABPC4

• NCBI Official Name:

  Polyadenylate-binding protein 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jonson, L., et al. Mol. Cell Proteomics 6 (5) :798-811 (2007)

• Background Reference 02:

  Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

• Background Reference 03:

  Olsen, J.V., et al. Cell 127 (3) :635-648 (2006)

• Background Reference 04:

  Rush, J., et al. Nat. Biotechnol. 23 (1) :94-101 (2005)

• Other Product Names:

  Polyadenylate-binding protein 4, PABP-4, Poly (A) -binding protein 4, Activated-platelet protein 1, APP-1, Inducible poly (A) -binding protein, iPABP, PABPC4, APP1, PABP4

• Tested Applications:

  WB

• Protein ID:

  12229875

• Physical Properties:

  Liquid