CPSF4 Antibody

• Background:

  Inhibition of the nuclear export of poly (A) -containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

• NCBI Gene ID:

  10898

• Swiss Prot:

  O95639

• Accession Number:

  EAW76662

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPSF4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CPSF4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  24 kDa, 28 kDa, 30 kDa

• Applications Notes:

  CPSF4 antibody can be used for detection of CPSF4 by ELISA at 1:62500. CPSF4 antibody can be used for detection of CPSF4 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CPSF4

• NCBI Official Name:

  Cleavage and polyadenylation specific factor 4, 30kDa

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CPSF4, CPSF30, NAR, NEB1

• Tested Applications:

  ELISA, WB

• Protein ID:

  119597068

• Physical Properties:

  Liquid