PDK4 Antibody

• Background:

  PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

• NCBI Gene ID:

  5166

• Swiss Prot:

  Q16654

• Accession Number:

  NP_002603

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Tranfected 293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDK4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  46 kDa

• Applications Notes:

  PDK4 antibody can be used for detection of PDK4 by ELISA at 1:312500. PDK4 antibody can be used for detection of PDK4 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PDK4

• NCBI Official Name:

  Pyruvate dehydrogenase kinase, isozyme 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kimura, K., (2006) Genome Res. 16 (1), 55-65.

• Other Product Names:

  PDK4, FLJ40832, PDHK4

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4505693

• Physical Properties:

  Liquid