PDK3 Antibody

• Background:

  PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

• NCBI Gene ID:

  5165

• Swiss Prot:

  Q15120

• Accession Number:

  NP_005382

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Tranfected 293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDK3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  47 kDa

• Applications Notes:

  PDK3 antibody can be used for detection of PDK3 by ELISA at 1:312500. PDK3 antibody can be used for detection of PDK3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PDK3

• NCBI Official Name:

  Pyruvate dehydrogenase kinase, isozyme 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Degenhardt, T., (2007) J. Mol. Biol. 372 (2), 341-355.

• Other Product Names:

  PDK3, CMTX6, GS1-358P8.4

• Tested Applications:

  ELISA, WB

• Protein ID:

  4885545

• Physical Properties:

  Liquid