PDK3 Antibody

• Background:

  The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  5165

• Swiss Prot:

  Q15120

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  Mouse kidney

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 47kDa

• Fragment:

  IgG

• Symbol:

  PDK3

• Positive Control 2:

  Mouse brain

• Positive Control 3:

  Mouse spleen

• Positive Control 4:

  Rat brain

• NCBI Official Name:

  Pyruvate dehydrogenase kinase, isozyme 3

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  PDK3, CMTX6, GS1-358P8.4

• Tested Applications:

  WB, IHC

• Physical Properties:

  Liquid