LIN28 Antibody

• Background:

  LIN28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. The association of LIN28 with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. IT binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. LIN28 is Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.

• NCBI Gene ID:

  79727

• Swiss Prot:

  Q9H9Z2

• Accession Number:

  NP_078950

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIN28.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store LIN28 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  23 kDa

• Applications Notes:

  LIN28 antibody can be used for detection of LIN28 by ELISA at 1:62500. LIN28 antibody can be used for detection of LIN28 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  LIN28A

• NCBI Official Name:

  Lin-28 homolog A (C. elegans)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gregory, S.G., (2006) Nature 441 (7091), 315-321.

• Other Product Names:

  LIN28, CSDD1, FLJ12457, LIN-28, LIN28A, ZCCHC1, LIN28, lin-28A

• Tested Applications:

  ELISA, WB

• Protein ID:

  13375938

• Physical Properties:

  Liquid