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LIN28B Antibody

CAT: 0223-79-728Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-728Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
NCBI Gene ID
389421
Swiss Prot
Q6ZN17
Accession Number
NP_001004317.1
Host
Rabbit
Reactivity
Human
Immunogen
LIN28B antibody was raised against a peptide sequence around aa.242~246 (P-S-V-Q-K) derived from Human LIN28B.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Stem Cell
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
21 32 kDa
Specificity
This antibody detects endogenous levels of total LIN28B protein.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
LIN28B
NCBI Official Name
Lin-28 homolog B (C. elegans)
NCBI Organism
Homo sapiens
Background Reference 01
"Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. "Guo Y., Chen Y., Ito H., et al. Gene 384:51-61 (2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. "Heo I., Joo C., Cho J., Ha M., Han J., Kim V. N. Mol. Cell 32:276-284 (2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation. "Heo I., Joo C., Kim Y. -K., Kim V. N., et al. Cell 138:696-708 (2009)
Other Product Names
CSDD2, CTNNB, OK/SW-cl.35, PRO2286, CSDD2, FLJ16517
Tested Applications
WB
Protein ID
51972214
Physical Properties
Liquid

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