RFC3 Antibody

• Background:

  The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

• NCBI Gene ID:

  5983

• Swiss Prot:

  P40938

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RFC3 (NP_002906.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity purification

• Positive Control:

  MCF-7

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 41kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  RFC3

• Positive Control 2:

  SW480

• Positive Control 3:

  HL-60

• Positive Control 4:

  NIH/3T3

• Positive Control 5:

  293T

• Positive Control 6:

  Mouse liver

• NCBI Official Name:

  Replication factor C (activator 1) 3, 38kDa

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  RFC3, MGC5276, RFC38

• Tested Applications:

  WB

• Physical Properties:

  Liquid