RFC5 Antibody

• Background:

  The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.

• NCBI Gene ID:

  5985

• Swiss Prot:

  P40937

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 181-340 of human RFC5 (NP_031396.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity purification

• Positive Control:

  HeLa

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 38kDa

• Fragment:

  IgG

• Symbol:

  RFC5

• Positive Control 2:

  U-251MG

• Positive Control 3:

  SKOV3

• Positive Control 4:

  MCF-7

• Positive Control 5:

  Mouse liver

• Positive Control 6:

  Mouse spleen

• NCBI Official Name:

  Replication factor C (activator 1) 5, 36.5kDa

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  RFC5, MGC1155, RFC36

• Tested Applications:

  WB, IHC

• Physical Properties:

  Liquid