RFC5 Antibody

• Background:

  The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

• NCBI Gene ID:

  5985

• Swiss Prot:

  P40937

• Accession Number:

  P40937

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human RFC5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  38 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  RFC5

• NCBI Official Name:

  Replication factor C subunit 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Stelzl, U., Cell 122 (6), 957-968 (2005)

• Background Reference 02:

  Andersen, J.S., Nature 433 (7021), 77-83 (2005)

• Other Product Names:

  Replication factor C subunit 5, Activator 1 36 kDa subunit, A1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5

• Tested Applications:

  WB, Flow

• Protein ID:

  728777

• Physical Properties:

  Liquid