Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]

Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]

• Description: The catalytic subunit α of protein kinase A is a key regulatory enzyme that in humans is encoded by the PRKACA gene. cAMP-dependent protein kinase catalytic subunit beta is an enzyme that in humans is encoded by the PRKACB gene. cAMP-dependent protein kinase catalytic subunit gamma is an enzyme that in humans is encoded by the PRKACG gene. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. Defective regulation of PKA holoenzyme activity has been linked to the progression of cardiovascular disease, certain endocrine disorders and cancers.
• Product Name Alternative: cAMP dependent protein kinase catalytic subunit alpha antibody cAMP dependent protein kinase catalytic subunit beta antibody cAMP dependent protein kinase catalytic subunit gamma antibody PKA C alpha antibody PKA C beta antibody PKA C gamma antibody PKACA antibody PRKACA antibody PRKACB antibody PRKACG antibody
• Abbreviation: PRKACA, PKACA, PRKACB, PRKACG
• CAS Number: 9000-83-3
• Swiss Prot: P17612 Human, P22694 Human, P22612 Human, P05132 Mouse, P27791 Rat
• Cellular Locus: Cytoplasm, Cell membrane, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C-gamma.
• Isotype: IgG
• Clone: JE45-17
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: NIH/3T3 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 40 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes