Phospho-PKA R2 (S99) Recombinant Rabbit Monoclonal Antibody [SC54-04]

Phospho-PKA R2 (S99) Recombinant Rabbit Monoclonal Antibody [SC54-04]

• Description: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
• Product Name Alternative: cAMP dependent protein kinase regulatory subunit alpha 2 antibody cAMP dependent protein kinase regulatory subunit RII alpha antibody cAMP dependent protein kinase type II alpha regulatory chain antibody cAMP dependent protein kinase type II alpha regulatory subunit antibody cAMP-dependent protein kinase type II-alpha regulatory subunit antibody KAP2 antibody KAP2_HUMAN antibody MGC3606 antibody PKR 2 antibody PKR2 antibody PRKA R2 antibody PRKAR 2 antibody PRKAR2 antibody PRKAR2A antibody Protein kinase A RII alpha subunit antibody Protein kinase cAMP dependent regulatory type II alpha antibody
• Abbreviation: PRKAR2A
• CAS Number: 9000-83-3
• Swiss Prot: P13861 Human, P12367 Mouse, P12368 Rat
• Cellular Locus: Cell membrane, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Pig
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126 / 404.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
• Positive Control: Hela, MCF-7, human spleen tissue, mouse testis tissue, mouse heart tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 46 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes