Phospho-VASP (S156) Recombinant Rabbit Monoclonal Antibody [JE45-91]

Phospho-VASP (S156) Recombinant Rabbit Monoclonal Antibody [JE45-91]

• Description: Vasodilator-stimulated phosphoprotein is a protein that in humans is encoded by the VASP gene. Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an N-terminal EVH1 domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions cell membranes. In the mid-region of the protein, family members have a proline-rich region that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
• Product Name Alternative: Vasodilator stimulated phosphoprotein antibody Vasodilator-stimulated phosphoprotein antibody VASP antibody VASP_HUMAN antibody
• Abbreviation: VASP
• CAS Number: 9000-83-3
• Swiss Prot: P50552 Human, P70460 Mouse,
• Cellular Locus: Cytoplasm, cytoskeleton, Cell junction, focal adhesion, tight junction, Cell projection, lamellipodium membrane, filopodium membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser156 of human VASP.
• Isotype: IgG
• Clone: JE45-91
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P
• Positive Control: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, A431 cells treated with 10μM forskolin for 1 hour, mouse spleen tissue, rat spleen tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 40 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes