Phospho-p53 (S376) Recombinant Rabbit Monoclonal Antibody [ST0440]

Phospho-p53 (S376) Recombinant Rabbit Monoclonal Antibody [ST0440]

• Description: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
• Product Name Alternative: Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody
• Abbreviation: TP53
• CAS Number: 9000-83-3
• Swiss Prot: P04637 Human
• Cellular Locus: Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human p53 aa 344-393 / 393.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate, RAW264.7 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 53 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes