Phospho-DRP1 (S616) Recombinant Rabbit Monoclonal Antibody [PSH06-77]

Phospho-DRP1 (S616) Recombinant Rabbit Monoclonal Antibody [PSH06-77]

• Description: Dynamin-1-like protein is a GTPase that regulates mitochondrial fission. In humans, dynamin-1-like protein, which is typically referred to as dynamin-related protein 1 (Drp1), is encoded by the DNM1L gene and is part of the dynamin superfamily (DSP) family of proteins. Mitochondria routinely undergo fission and fusion events that maintain a dynamic reticular network. Drp1 is a fundamental component of mitochondrial fission. Indeed, Drp1 deficient neurons have large, strongly interconnected mitochondria due to dysfunctional fission machinery. Fission helps facilitate mitophagy, which is the breakdown and recycling of damaged mitochondria. Dysfunction in the DRP activity may result in mutated DNA or malfunctioning proteins diffusing throughout the mitochondrial system. In addition, fission results in fragmented mitochondria more capable of producing of reactive oxygen species, which can disrupt normal biochemical processes inside of cells. ROS can be formed from incomplete transfer of electrons through the electron transport chain. Furthermore, fission influences calcium flux within the cell, linking Drp1 to apoptosis and cancer.
• Product Name Alternative: DLP1 antibody dnm1l antibody DNM1L_HUMAN antibody Dnm1p/Vps1p-like protein antibody dnml1 antibody DRP1 antibody DVLP antibody Dymple antibody Dynamin 1 like antibody Dynamin family member proline-rich carboxyl-terminal domain less antibody Dynamin like protein antibody Dynamin related protein 1 antibody Dynamin-1-like protein antibody Dynamin-like protein 4 antibody Dynamin-like protein antibody Dynamin-like protein IV antibody Dynamin-related protein 1 antibody DYNIV 11 antibody EMPF antibody EMPF1 antibody FLJ41912 antibody HdynIV antibody VPS1 antibody
• Abbreviation: DNM1L, DLP1, DRP1
• CAS Number: 9000-83-3
• Swiss Prot: O00429 Human, Q8K1M6 Mouse, O35303 Rat
• Cellular Locus: Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within
• Isotype: IgG
• Clone: PSH06-77
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Tissue, IF-Cell, FC
• Positive Control: HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL nocodazole for 18 hours cell lysate, HeLa, C2C12 cell lysate, PC-12 cell lysate, mouse brain tissue, Jurkat cell lysate, HEK-293 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, rat brain tissue, PC-12.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:5,000 ;IHC-P: 1:10,000 ;IF-Tissue: 1:500-1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 82 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes