DRP1 Rabbit Polyclonal Antibody

Dynamin-1-like protein is a GTPase that regulates mitochondrial fission. In humans, dynamin-1-like protein, which is typically referred to as dynamin-related protein 1 (Drp1), is encoded by the DNM1L gene and is part of the dynamin superfamily (DSP) family of proteins. Mitochondria routinely undergo fission and fusion events that maintain a dynamic reticular network. Drp1 is a fundamental component of mitochondrial fission. Indeed, Drp1 deficient neurons have large, strongly interconnected mitochondria due to dysfunctional fission machinery. Fission helps facilitate mitophagy, which is the breakdown and recycling of damaged mitochondria. Dysfunction in the DRP activity may result in mutated DNA or malfunctioning proteins diffusing throughout the mitochondrial system. In addition, fission results in fragmented mitochondria more capable of producing of reactive oxygen species, which can disrupt normal biochemical processes inside of cells. ROS can be formed from incomplete transfer of electrons through the electron transport chain. Furthermore, fission influences calcium flux within the cell, linking Drp1 to apoptosis and cancer. Several studies have indicated that Drp1 is essential for proper embryonic development. Drp1 knockout mice exhibit abnormal brain development and die around embryonic day 12. In neural specific Drp1 knockout mice, brain size is reduced and apoptosis is increased. Synapse formation and neurite growth are also impaired. A second group of researchers generated another neural specific knockout mouse line. They found that knocking out Drp1 resulted in the appearance of large mitochondria in Purkinje cells and prevented neural tube formation. In humans, loss of Drp1 function affects brain development and is also associated with early mortality.

9007-83-4

DLP1 antibody dnm1l antibody DNM1L_HUMAN antibody Dnm1p/Vps1p-like protein antibody dnml1 antibody DRP1 antibody DVLP antibody Dymple antibody Dynamin 1 like antibody Dynamin family member proline-rich carboxyl-terminal domain less antibody Dynamin like protein antibody Dynamin related protein 1 antibody Dynamin-1-like protein antibody Dynamin-like protein 4 antibody Dynamin-like protein antibody Dynamin-like protein IV antibody Dynamin-related protein 1 antibody DYNIV 11 antibody EMPF antibody EMPF1 antibody FLJ41912 antibody HdynIV antibody VPS1 antibody

DNM1L, DLP1, DRP1

O00429 Human, Q8K1M6 Mouse, O35303 Rat

Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

Recombinant protein within human DRP1 aa 1-736 / 736.

Non-conjugated

Rabbit polyclonal Antibody

WB, IF-Cell, FC

A549 cell lysate, 293 cell lysate, HCT116 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, NIH/3T3, 293T.

1 mg/mL

WB: 1:1,000;IF-Cell: 1:200;FC: 1:500-1:1,000

Protein A affinity purified.

Liquid

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Predicted band size: 82 kDa

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.