TIMP-1

• Description :

  TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

• Synonyms :

  TIMP1; EPA; EPO; HCI; CLGI; TIMP

• NCBI Gene ID :

  7076

• UniProt :

  P01033

• Accession Number :

  NP_003245

• Accession Number mRNA :

  NM_003254

• Chromosomal Location :

  Xp11.3-p11.23

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Human recombinant TIMP-1 protein

• Clone :

  (#4A16)

• Applications :

  WB, IHC (P)

• Purification Method :

  Protein G chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source :

  Mouse

• Isotype :

  IgG2