TIMP-1 Antibody

• Description:

  This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

• Specifications:

  Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

• UniProt:

  P01033

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 157-188 from the human protein was used as the immunogen for the TIMP-1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  SAS precipitation

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the TIMP-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This TIMP-1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the TIMP-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the TIMP-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of fixed and permeabilized human A2058 cells with TIMP-1 antibody (green) and anti-Actin (red) .