CD3 FITC / CD19 PE / CD45 Cy5-PE[CD3 / CD19 PE / CD45 Cy5]
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Catalog number
MBS396669
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Price
Please ask
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Size
NA
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Products_type
Antibody
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Reactivity
Human
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Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Cy5.5, Anti-FITC Antibody
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Gene target
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Gene symbol
CD19, PTPRCAP, PTPRC
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Short name
CD3 FITC / CD19 PE / CD45 -PE[CD3 / CD19 PE / CD45 ]
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Technique
FITC, Fluorescein
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Host
Mouse
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Label
FITC
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Alternative name
CD3 fluorecein / CD19 molecule peroxidase / CD45 cyanine 5-peroxidase[CD3 / CD19 molecule peroxidase / CD45 cyanine 5]
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Alternative technique
fluorescine
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Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
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Gene info
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Identity
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Gene
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Long gene name
CD19 molecule
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Synonyms gene name
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Locus
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Discovery year
1991-06-04
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
- Minor histocompatibility antigens
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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