KOMBITEST CD3 FITC / CD8 PE / CD45 PerCP

Description

Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
Properties

This Exbio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exbio FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
Conjugate

Peridinin chlorophyll (PerCP)

Gene target

KOMBITEST CD3 CD8 CD45
Gene symbol

CD8A, PTPRCAP, PTPRC
Short name

KOMBITEST CD3 FITC / CD8 PE / CD45 PerCP
Technique

Percp, FITC, Fluorescein, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the KOMBITEST CD3 FITC / CD8 PE / CD45 PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Exbio.
Label

FITC
Alternative name

KOMBITEST CD3 fluorecein / CD8 peroxidase / CD45 PerCP
Alternative technique

antibodies-percp, fluorescine

Identity

HGNC:1706
Gene

CD8A
Long gene name

CD8a molecule
Synonyms gene
- CD8
Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
Locus

2p11.2
Discovery year

1986-01-01
Entrez gene record

925
Pubmed identfication
- 1541829
RefSeq identity
- NM_001768
Classification
- V-set domain containing
- CD molecules
VEGA ID

OTTHUMG00000130265
Locus Specific Databases
- CD8Abase: Mutation registry for CD8 deficiency
- LRG_44

Identity

HGNC:9666
Gene

PTPRC
Long gene name

protein tyrosine phosphatase receptor type C
Synonyms gene
- CD45
Synonyms
- LCA
- T200
- GP180
GenBank acession
- Y00062
Locus

1q31.3-q32.1
Discovery year

1986-01-01
Entrez gene record

5788
Pubmed identfication
- 2169617
RefSeq identity
- NM_002838
Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
VEGA ID

OTTHUMG00000035702
Locus Specific Databases
- PTPRCbase: Mutation registry for CD45 deficiency
- LRG_95

Name

CD4 Lymphocyte Count
Concept

Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data