CD3 FITC / CD19 PE / CD45 Cy5-PE
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Catalog number
MBS396669
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Price
Please ask
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Size
100 Tests
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Other size
please contact us to order other different size
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Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Cy5.5, Anti-FITC Antibody
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Gene target
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Gene symbol
CD19, PTPRCAP, PTPRC
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Short name
CD3 FITC / CD19 PE / CD45 -PE
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Technique
FITC, Fluorescein
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Label
FITC
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Alternative name
CD3 fluorecein / CD19 molecule peroxidase / CD45 cyanine 5-peroxidase
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Alternative technique
fluorescine
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Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
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Gene info
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Identity
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Gene
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Long gene name
CD19 molecule
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Synonyms gene name
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Locus
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Discovery year
1991-06-04
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
- Minor histocompatibility antigens
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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