-
Category
Three-Color Reagents (FITC/RPE/Cy5RPE)
-
Long description
Use Exalpha's 3 color CD markers to add flexibility to your current single laser system capabilities. The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. CD3 indentifies human T cells expressing the 22-28,000 M.W surface antigen. CD19 identifies human B cells associated with 10% of peripheral blood lymphocytes. CD19 identifies lymphocytes expressing the 95,000 M.W. surface antigen. CD45 identifies all human leucocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinphils and basophils in peripheral blood expressing the 180-220K M.W. T200 family surface antigen. CD45 is also present on thymus,spleen, tonsil and progenitor cells in bone marrow.
-
Antibody come from
CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
-
Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Affinity chromatography
-
Clone
not specified
-
Antigen antibody binding interaction
Mouse anti CD3 antibody conjugated to FITC / CD19 antibody conjugated to PE / CD45 antibody conjugated to Cy5-PE Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Flow Cytometry
-
Antibody s suited for
PBMC: Add 10 µl of Exalph's 3 color antibody reagent/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C.Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of Exalpha's 3 color antibody reagent MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
-
Storage
4ºC
-
Relevant references
1.Orfao A, Gonz‡lez de Buitrago JM La citometr’a de flujo en el laboratorio cl’nico. Sociedad espa–ola de bioqu’mica cl’nica y patolog’a Molecular 1995._x000B_2. Stetler-Stevenson M. Flow cytometry analysis of lymphomas and lymphoproliferative disorders. Semin Hematol 2001 Apr;38(2):111-23._x000B_3.MenŽndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)_x000B_4.GratamaJW, MenŽndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)_x000B_5.Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2._x000B_6.Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5._x000B_7.Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A._x000B_8.Gorczyza W. et al. An approach to diagnosis of T-cell lymphoprolipherative disorders by flow cytometry. Cytometry (Clinical cytometry) 50:177-190 (2002)_x000B_9.Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Cytometry 46: 23-7 (2001)_x000B_10.Jennings CD, Foon KA. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy. Blood 90(8): 2863-2892 (1997)_x000B_11.Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991) _x000B_12.Prince HK et al. Influence of racial background on the distribution of T-cell subsets and Leu-11 positive lymphocytes in healthy_x000B_blood donors. Diagn Immunol. 3: 33-39 (1985) _x000B_13.Kotylo PK et al. Reference ranges for lymphocyte subsets in pediatric patients. Am J Clin Pathol 100:111-5 (1993)
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
-
Conjugation
Cy5.5, Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD19, PTPRCAP, PTPRC
-
Short name
Mouse anti CD3 antibody conjugated FITC / CD19 antibody conjugated PE / CD45 antibody conjugated -PE
-
Technique
Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
-
Host
mouse
-
Isotype
not specified
-
Label
FITC and PE and Cy5-PE
-
Species
Mouse, Mouses
-
Alternative name
Mouse antibody to CD3 (antibody to-) coupled to fluorecein / CD19 molecule (antibody to-) coupled to peroxidase / CD45 (antibody to-) coupled to cyanine 5-peroxidase
-
Alternative technique
antibodies, murine, fluorescine
-
Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
-
