CD3 FITC / CD19 PE / CD45 Cy5-PE, Three-Color Reagents (FITC/RPE/Cy5RPE)

• Catalog number
  X1066
• Price
  Please ask
• Size
  100Tests

• Host Source
  Mouse
• Clone Name
  NA
• Product Category
  Flow Cytometry
• Species Reactivity
  Human
• Application
  Flow Cytometry
• Immunogen
  CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
• Purification Method
  Affinity chromatography
• Shipping Conditions
  Room Temperature
• Storage conditions
  CD3 FITC / CD19 PE / CD45 Cy5-PE, Three-Color Reagents (FITC/RPE/Cy5RPE) should be stored at 4-8ºC. DO NOT FREEZE
• Datasheet link
• Properties
  Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
• Conjugation
  Cy5.5, Anti-FITC Antibody

• Gene target
  CD3   CD19   CD45   Three-Color   Reagents   RPE/Cy5RPE  
• Gene symbol
  RPE, CD19, PTPRCAP, PTPRC
• Short name
  CD3 FITC / CD19 PE / CD45 -PE, Three-Color Reagents (FITC/RPE/Cy5RPE)
• Technique
  FITC, Fluorescein
• Isotype
  NA
• Label
  FITC
• Alternative name
  CD3 fluorecein / CD19 molecule peroxidase / CD45 cyanine 5-peroxidase, Three-Color Reagents (fluorecein/RPE/Cy5RPE)
• Alternative technique
  fluorescine
• Alternative to gene target
  CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and

Gene info

• Identity
  HGNC:10293
• Gene
  RPE
• Long gene name
  ribulose-5-phosphate-3-epimerase
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  6120
• RefSeq identity
  • NM_006916
• VEGA ID
  OTTHUMG00000154690

Gene info

• Identity
  HGNC:1633
• Gene
  CD19
• Long gene name
  CD19 molecule
• Synonyms gene name
  • CD19 antigen
• Locus
  16p11.2
• Discovery year
  1991-06-04
• Entrez gene record
  930
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000097049
• Locus Specific Databases
  • CD19base: Mutation registry for CD19 deficiency
  • LRG_35

Gene info

• Identity
  HGNC:9667
• Gene
  PTPRCAP
• Long gene name
  protein tyrosine phosphatase receptor type C associated protein
• Synonyms
  • LPAP
  • CD45-AP
• Locus
  11q13.2
• Discovery year
  1996-04-22
• Entrez gene record
  5790
• RefSeq identity
  • NM_005608
• VEGA ID
  OTTHUMG00000150325

Gene info

• Identity
  HGNC:9666
• Gene
  PTPRC
• Long gene name
  protein tyrosine phosphatase receptor type C
• Synonyms gene
  • CD45
• Synonyms
  • LCA
  • T200
  • GP180
• GenBank acession
  • Y00062
• Locus
  1q31.3-q32.1
• Discovery year
  1986-01-01
• Entrez gene record
  5788
• Pubmed identfication
  • 2169617
• RefSeq identity
  • NM_002838
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000035702
• Locus Specific Databases
  • PTPRCbase: Mutation registry for CD45 deficiency
  • LRG_95

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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