CD3 FITC / CD19 PE / CD45 Cy5-PE, Three-Color Reagents (FITC/RPE/Cy5RPE)

• Catalog number
  X1066S
• Price
  Please ask
• Size
  50Tests

• Host Source
  Mouse
• Clone Name
  NA
• Product Category
  Flow Cytometry
• Species Reactivity
  Human
• Application
  Flow Cytometry
• Immunogen
  CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
• Purification Method
  Affinity Chromatography
• Shipping Conditions
  Room Temperature
• Storage conditions
  CD3 FITC / CD19 PE / CD45 Cy5-PE, Three-Color Reagents (FITC/RPE/Cy5RPE) should be stored at 4-8ºC. DO NOT FREEZE
• Datasheet link
• Properties
  Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
• Conjugation
  Cy5.5, Anti-FITC Antibody

• Gene target
  CD3   CD19   CD45   Three-Color   Reagents   RPE/Cy5RPE  
• Gene symbol
  RPE, CD19, PTPRCAP, PTPRC
• Short name
  CD3 FITC / CD19 PE / CD45 -PE, Three-Color Reagents (FITC/RPE/Cy5RPE)
• Technique
  FITC, Fluorescein
• Isotype
  NA
• Label
  FITC
• Alternative name
  CD3 fluorecein / CD19 molecule peroxidase / CD45 cyanine 5-peroxidase, Three-Color Reagents (fluorecein/RPE/Cy5RPE)
• Alternative technique
  fluorescine
• Alternative to gene target
  CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and

Gene info

• Identity
  HGNC:10293
• Gene
  RPE
• Long gene name
  ribulose-5-phosphate-3-epimerase
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  6120
• RefSeq identity
  • NM_006916
• VEGA ID
  OTTHUMG00000154690

Gene info

• Identity
  HGNC:1633
• Gene
  CD19
• Long gene name
  CD19 molecule
• Synonyms gene name
  • CD19 antigen
• Locus
  16p11.2
• Discovery year
  1991-06-04
• Entrez gene record
  930
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000097049
• Locus Specific Databases
  • CD19base: Mutation registry for CD19 deficiency
  • LRG_35

Gene info

• Identity
  HGNC:9667
• Gene
  PTPRCAP
• Long gene name
  protein tyrosine phosphatase receptor type C associated protein
• Synonyms
  • LPAP
  • CD45-AP
• Locus
  11q13.2
• Discovery year
  1996-04-22
• Entrez gene record
  5790
• RefSeq identity
  • NM_005608
• VEGA ID
  OTTHUMG00000150325

Gene info

• Identity
  HGNC:9666
• Gene
  PTPRC
• Long gene name
  protein tyrosine phosphatase receptor type C
• Synonyms gene
  • CD45
• Synonyms
  • LCA
  • T200
  • GP180
• GenBank acession
  • Y00062
• Locus
  1q31.3-q32.1
• Discovery year
  1986-01-01
• Entrez gene record
  5788
• Pubmed identfication
  • 2169617
• RefSeq identity
  • NM_002838
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000035702
• Locus Specific Databases
  • PTPRCbase: Mutation registry for CD45 deficiency
  • LRG_95

MeSH Data

• Name
  CD4 Lymphocyte Count
• Concept
  Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
• Tree numbers
  • E01.370.225.500.195.107.595.500.150
  • E01.370.225.625.107.595.500.150
  • E05.200.500.195.107.595.500.150
  • E05.200.625.107.595.500.150
  • E05.242.195.107.595.500.150
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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