Anti- Flag-Tag Antibody
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Catalog numberGEN8502277
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PricePlease ask
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Size100ug
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Long nameFlag-Tag Mouse Monoclonal Antibody(2C5)-HRP conjugated
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AntigenFlag-Tag
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Synonim namesN/A
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Other namesN/A
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Gene nameFlag-Tag
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Gene synonimsN/A
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CategoryAntibodies
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ClonalityN/A
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Clone2C5
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Reacts withDue to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
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SpecificityThe Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. ;This is an antibody designed to detect Flag-Tag
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Purification methodThe most common purification methods used in the antibody production are Affinity Chromatography, Caprylic Acid Ammonium Sulfate Precipitation, Antigen Affinity method, etc.
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Form AppearanceMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Concentration100ug/100ul
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Storage shipping and handlingStore at -20 degree C for 1 year
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ApplicationsWestern Blot (WB)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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French translationanticorps
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Gene target
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Gene symbolTRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
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Short nameAnti-Flag-Tag
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TechniqueAntibody, anti, antibody to, antibodies against human proteins, antibodies for, Tags
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HostMouse (Mus musculus)
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IsotypeN/A
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LabelFlag
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Alternative nameFlag-Tag Antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 15 (anticodon UAG)
- transfer RNA-Leu (TAG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 7 (anticodon UAG)
- transfer RNA-Leu (TAG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 40 (anticodon UAG) pseudogene
- transfer RNA-Leu (TAG) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data