Mouse Flag Tag antibody
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Catalog number10R-10459
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PricePlease ask
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Size100 ug
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ApplicationsELISA, WB
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Product TypePrimary Antibodies
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Product SubtypeMonoclonal Antibodies
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Research AreaMiscellaneous
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ImmunogenFlag Tag antibody was raised in Mouse using a synthetic peptide (KLH-coupled) corresponding to the residues of Flag Tag protein as the immunogen
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SpecificityFlag Tag antibody recognizes Flag and Flag-tagged fusion proteins
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Cross ReactivityNA
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CloneM78828
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Concentration1 mg/ml
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Form BufferSupplied in citrate-tris-HCl buffer, pH 7.0 with 0.02% Proclin 300
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StorageStore at -20 deg C for 1 year, store at 2-8 deg C for up to 1 month
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Shipping InfoBlue Ice
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolTRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
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Short nameMouse Tag antibody
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TechniqueAntibody, Mouse, antibodies against human proteins, antibodies for, mouses, Tags
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HostMouse
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IsotypeIgG1
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LabelFlag
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SpeciesMouse, Mouses
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Alternative nameMouse monoclonal Flag Tag antibody
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 15 (anticodon UAG)
- transfer RNA-Leu (TAG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 7 (anticodon UAG)
- transfer RNA-Leu (TAG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 40 (anticodon UAG) pseudogene
- transfer RNA-Leu (TAG) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
