FLAG Tag Antibody

• Catalog number
  bs-0965R
• Price
  Please ask
• Size
  0.1ml

• Long name
  FLAG Tag Primary Polyclonal Antibody
• Also known as
  FLAG Tag PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  FLAG Tag
• Specificity
  This is a highly specific antibody against FLAG Tag.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  MAP of Flag Tag peptide
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Tags
• Cross reactive species details
  FLAG Tag
• Background of the target antigen
  This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; flag; DDDDK tag N-T.
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Additional conjugation
  Flag
• French translation
  anticorps

• Gene target
  Tag  
• Gene symbol
  TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
• Short name
  Anti-FLAG Tag
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Label
  Flag
• Alternative name
  FLAG Tag Polyclonal Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:34713
• Gene
  TRL-TAG2-1
• Long gene name
  tRNA-Leu (anticodon TAG) 2-1
• Synonyms gene
  • TRNAL15
• Synonyms gene name
  • transfer RNA leucine 15 (anticodon UAG)
  • transfer RNA-Leu (TAG) 2-1
• Synonyms
  • tRNA-Leu-TAG-2-1
• GenBank acession
  • HG983905
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189151
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34591
• Gene
  TRL-TAG3-1
• Long gene name
  tRNA-Leu (anticodon TAG) 3-1
• Synonyms gene
  • TRNAL7
• Synonyms gene name
  • transfer RNA leucine 7 (anticodon UAG)
  • transfer RNA-Leu (TAG) 3-1
• Synonyms
  • tRNA-Leu-TAG-3-1
• GenBank acession
  • HG983906
• Locus
  16p12.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189032
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35011
• Gene
  TRL-TAG4-1
• Long gene name
  tRNA-Leu (anticodon TAG) 4-1
• Synonyms gene
  • TRNAL40P
• Synonyms gene name
  • transfer RNA leucine 40 (anticodon UAG) pseudogene
  • transfer RNA-Leu (TAG) 4-1
• Synonyms
  • tRNA-Leu-TAG-4-1
• GenBank acession
  • HG983907
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189441
• RefSeq identity
  • NG_008497
• Classification
  • Cytoplasmic transfer RNA pseudogenes

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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