FLAG Tag antibody

  • Catalog number
    10R-7750
  • Price
    Please ask
  • Size
    500 µg
  • Category
    Primary Antibody
  • Antibody Subtype
    Monoclonal Antibodies
  • Area of research
    Miscellaneous
  • Type of Immunogen
    FLAG Tag antibodies were raised in Mouse using Flag-conjugated proteins ..
  • Raised in
    Mouse
  • Specificity
    Human FLAG Tag
  • Isotypes
    IgG1 kappa
  • Clone
    NYRFLAG
  • Method of Purification
    FLAG Tag antibody was purified by Protein A and Ion exchange chromatography
  • Form Buffer
    Provided in lyophilized form, reconstitute in sterile H2O or PBS buffer
  • Storage
    Store at 4 deg C for short term storage. For long term storage aliquot and store at -20 deg C, avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; IHC; WB
  • Usage Recommendations
    ELISA: 1:20,000, WB: 1 ug/ml
  • URL
  • Properties
    If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • French translation
    anticorps
  • Gene target
    Tag  
  • Gene symbol
    TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
  • Short name
    Tag antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Tags
  • Label
    Flag
  • Alternative name
    Mouse monoclonal FLAG Tag antibody
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 2-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 15 (anticodon UAG)
    • transfer RNA-Leu (TAG) 2-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 3-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 7 (anticodon UAG)
    • transfer RNA-Leu (TAG) 3-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 4-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 40 (anticodon UAG) pseudogene
    • transfer RNA-Leu (TAG) 4-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Cytoplasmic transfer RNA pseudogenes
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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