Flag Tag

• Catalog number
  70R-36938
• Price
  Please ask
• Size
  100 µg

• Category
  Primary Antibody
• Antibody Subtype
  Polyclonal Antibodies, Purified
• Area of research
  Miscellaneous
• Type of Immunogen
  Flag Tag was raised in Rabbit using synthesized peptide containing the Flag epitope (D-Y-K-D-D-D-D-K) ..
• Raised in
  Rabbit
• Specificity
  Flag Tag detects Flag Tagged proteins overexpressed in cells The antibody recognizes the Flag Tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells
• Isotypes
  IgG
• Method of Purification
  Flag Tag was purified by affinity chromatography
• Concentration
  1 mg/ml
• Form Buffer
  Provided in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% NaN3 and 50% glycerol
• Storage
  Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles
• Shipping conditions
  Blue Ice
• Tested for
  WB
• Usage Recommendations
  WB: 1:500-1:1000
• URL
• More info
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag

• Gene target
  Tag  
• Gene symbol
  TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
• Short name
  Tag
• Technique
  Tags
• Label
  Flag
• Alternative name
  Rabbit Polyclonal Flag Tag

Gene info

• Identity
  HGNC:34713
• Gene
  TRL-TAG2-1
• Long gene name
  tRNA-Leu (anticodon TAG) 2-1
• Synonyms gene
  • TRNAL15
• Synonyms gene name
  • transfer RNA leucine 15 (anticodon UAG)
  • transfer RNA-Leu (TAG) 2-1
• Synonyms
  • tRNA-Leu-TAG-2-1
• GenBank acession
  • HG983905
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189151
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34591
• Gene
  TRL-TAG3-1
• Long gene name
  tRNA-Leu (anticodon TAG) 3-1
• Synonyms gene
  • TRNAL7
• Synonyms gene name
  • transfer RNA leucine 7 (anticodon UAG)
  • transfer RNA-Leu (TAG) 3-1
• Synonyms
  • tRNA-Leu-TAG-3-1
• GenBank acession
  • HG983906
• Locus
  16p12.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189032
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35011
• Gene
  TRL-TAG4-1
• Long gene name
  tRNA-Leu (anticodon TAG) 4-1
• Synonyms gene
  • TRNAL40P
• Synonyms gene name
  • transfer RNA leucine 40 (anticodon UAG) pseudogene
  • transfer RNA-Leu (TAG) 4-1
• Synonyms
  • tRNA-Leu-TAG-4-1
• GenBank acession
  • HG983907
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189441
• RefSeq identity
  • NG_008497
• Classification
  • Cytoplasmic transfer RNA pseudogenes

Similar products