FLAG Monoclonal Tag Antibody [6F7]

• Catalog number
  A26003-1
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  FLAG Monoclonal Tag Antibody
• Clone
  6F7
• Conjugation additive
  Unconjugated
• Product type
  Antibody
• Clonality
  monoclonal antibody
• Long name
  FLAG Monoclonal Tag Antibodies [6F7]
• Description
  FLAG Monoclonal Tag Antibody. Monoclonal antibodies ensure a highly specific binding to a certain epitope of the antigen and are much more LOT-to-LOT consistent than polyclonal antibodies.
• Storage conditions
  For short term storage the FLAG Monoclonal Tag Antibody [6F7] can be kept refrigerated at +4 degrees Celsius. For long term storage keep frozen at -20 degrees Celsius. Be sure to refer to the technical sheet for any additional specifications and requirements for proper storing of the antibodies.
• Tips
  FLAG Monoclonal Tag Antibody [6F7] should not be exposed to cycles of freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.
• Properties
  If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Monoclonal   Tag   [6F7]  
• Short name
  Anti-FLAG Monoclonal Tag Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
• Label
  Flag
• Alternative name
  FLAG Monoclonal Tag Antibody [6F7]
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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