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Category
Primary Antibodies
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Long description
Epitope tags are a useful way to identify protiens in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with ExalphaÕs DYKDDDDK FLAG antibody may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of this epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
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Antibody come from
Synthetic peptide from RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
FG4R
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Antigen antibody binding interaction
anti FLAG (DYKDDDDK) Tag Antibody
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Antibody is raised in
see techfile
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Antibody s reacts with
FLAG tagged fusion proteins
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
WB, ELISA, IP,IS, Dot Blot
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Antibody s suited for
Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins._x000B_WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)_x000B_IS: 1:500-2000 dilution._x000B_In order to obtain best results in other assays (e.g. Dot, ELISA, IP, etc.), optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
