Anti- Flag-Tag Antibody

• Catalog number
  GEN8502417
• Price
  Please ask
• Size
  100ug

• Long name
  Flag-Tag Mouse Monoclonal Antibody(2C5)
• Antigen
  Flag-Tag
• Synonim names
  N/A
• Other names
  N/A
• Gene name
  Flag-Tag
• Gene synonims
  N/A
• Category
  Antibodies
• Clonality
  N/A
• Clone
  2C5
• Reacts with
  Due to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
• Specificity
  The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. ;This is an antibody designed to detect Flag-Tag
• Purification method
  The most common purification methods used in the antibody production are Affinity Chromatography, Caprylic Acid Ammonium Sulfate Precipitation, Antigen Affinity method, etc.
• Form Appearance
  Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration
  100ug/100ul
• Storage shipping and handling
  Store at -20 degree C for 1 year
• Applications
  Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Clonagen antibody
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• French translation
  anticorps

• Gene target
  Tag  
• Gene symbol
  TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
• Short name
  Anti-Flag-Tag
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for, Tags
• Host
  N/A
• Isotype
  N/A
• Label
  Flag
• Alternative name
  Flag-Tag Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:34713
• Gene
  TRL-TAG2-1
• Long gene name
  tRNA-Leu (anticodon TAG) 2-1
• Synonyms gene
  • TRNAL15
• Synonyms gene name
  • transfer RNA leucine 15 (anticodon UAG)
  • transfer RNA-Leu (TAG) 2-1
• Synonyms
  • tRNA-Leu-TAG-2-1
• GenBank acession
  • HG983905
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189151
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34591
• Gene
  TRL-TAG3-1
• Long gene name
  tRNA-Leu (anticodon TAG) 3-1
• Synonyms gene
  • TRNAL7
• Synonyms gene name
  • transfer RNA leucine 7 (anticodon UAG)
  • transfer RNA-Leu (TAG) 3-1
• Synonyms
  • tRNA-Leu-TAG-3-1
• GenBank acession
  • HG983906
• Locus
  16p12.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189032
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35011
• Gene
  TRL-TAG4-1
• Long gene name
  tRNA-Leu (anticodon TAG) 4-1
• Synonyms gene
  • TRNAL40P
• Synonyms gene name
  • transfer RNA leucine 40 (anticodon UAG) pseudogene
  • transfer RNA-Leu (TAG) 4-1
• Synonyms
  • tRNA-Leu-TAG-4-1
• GenBank acession
  • HG983907
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189441
• RefSeq identity
  • NG_008497
• Classification
  • Cytoplasmic transfer RNA pseudogenes

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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