FLAG Tag antibody
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Catalog number70R-10687
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PricePlease ask
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Size1 ml
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CategoryPrimary Antibody
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Antibody SubtypePolyclonal Antibodies, Purified
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Area of researchDNA & RNA
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Type of ImmunogenFLAG antibodies were raised in Rabbit using DYKDDDDK-C conjugated with R-PE ..
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Raised inRabbit
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SpecificityHuman
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IsotypesIgG
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Method of PurificationProtein A purified total IgG
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Concentration2 mg/ml
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Form BufferProvided in 10 mM PO4, 150 mM NaCl, pH 7.4 with 1% BSA, 0.05% NaN3.
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StorageStore at 2-8 deg C
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Shipping conditionsBlue Ice
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Tested forDot Blot; WB; ELISA; IHC
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Usage RecommendationsELISA: 1:10000-1:50000; IHC: 1:50-1:200
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Assay InformationThis FLAG Tag antibody is also available in a pre-diluted ready-to-use format expecially for IHC under catalog no. 75R-1059
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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French translationanticorps
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Gene target
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Gene symbolTRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
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Short nameTag antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for, Tags
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LabelFlag
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Alternative nameRabbit polyclonal FLAG antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 15 (anticodon UAG)
- transfer RNA-Leu (TAG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 7 (anticodon UAG)
- transfer RNA-Leu (TAG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 40 (anticodon UAG) pseudogene
- transfer RNA-Leu (TAG) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data