EZClick™ Myristoylated Protein Assay Kit, Red Fluorescence

• Catalog number
  K177-100
• Price
  Please ask
• Size
  100 assays

• Description
  for labeling and detection of myristoylated proteins
• Summary
  BioVision EZClick™ Myristoylated Protein Assay Kit is a highly specific, simple and robust tool for labeling and detection of myristoylated proteins. In this assay, an Alkynyl Myristic Acid is added directly to the cells that get incorporated into proteins during or post translation followed by the click reaction with the azide-containing dye. This system offers a powerful method for imaging localization, trafficking and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
• Detection Method
  FACS and Microscopy
• Species Reactivity
  Mammalian
• Applications
  Detection and quantification of biosynthesis, dynamics and turnover of myristic acid modified proteins, Identification and localization of myristic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications
• Sample Type
  Suspension or adherent cell cultures
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Myristoylation is a co- and post-translational modification of proteins. Myristoylated proteins are involved in cellular trafficking, cellular transformation and oncogenesis. In this modification, Myristic acid, a saturated fatty acid is covalently conjugated to a protein through the action of N-myristoyl transferase (NMT). NMT transfers the myristate moiety from Myristoyl-CoA to an N-terminal glycine of a target protein. The NMT enzyme is associated with carcinogenesis. The upregulation of its expression and activity is observed in colorectal cancer, gall-bladder cancer, brain tumor etc. BioVision offers EZClick™ Myristoylated Protein Assay Kit, a highly specific, simple and robust tool for labeling and detection of myristoylated proteins. In this assay, an Alkynyl Myristic Acid is added directly to the cells that get incorporated into proteins during or post translation followed by the click reaction with the azide-containing dye. This system offers a powerful method for imaging localization, trafficking and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• Conjugation
  red dye

• Gene target
  EZClick™   Myristoylated   Protein   Kit   Red  
• Short name
  EZClick™ Myristoylated Protein Assay Kit, Red
• Technique
  Assay, assays
• Host
  Assay
• Label
  Red
• Alternative name
  EZClick™ Myristoylated Protein test reagent, Red Fluorescence
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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