EZClick™ Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

• Catalog number
  K462-100
• Price
  Please ask
• Size
  100 assays

• Description
  Microplate-based assay to detect total RNA content using metabolic labeling techniques
• Summary
  • Detection method- Fluorescence (Ex/Em= 494/521 nm) • Sample type- Adherent and suspension cells • Species reactivity- Mammalian • Applications- This assay provides a convenient and accurate procedure to detect and quantify RNA transcription in vitro and in vivo
• Detection Method
  Fluorescence (Ex/Em= 494/521 nm)
• Species Reactivity
  Eukaryotes
• Applications
  This assay provides a convenient and accurate procedure to detect and quantify RNA transcription in vitro and in vivo
• Sample Type
  Adherent and suspension cells
• Features Benefits
  Simple, fast, does not require lengthy incubation times
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  RNA plays crucial role in coding, decoding and regulation of genes, and protein expression in all living cells. The ability to detect newly synthesized RNA or changes in RNA levels under various physiological conditions, or resulting from disease, environmental damage, or drug treatments is an important aspect of toxicological profiling. Many anti-cancer drugs inhibit transcription, and most transcription inhibitors have useful pharmacological properties. BioVision’s EZClick™ Global RNA Synthesis Assay Kit provides a simple and robust tool for detection of global RNA transcription, temporally and spatially, or changes in RNA levels directly in living cells. De novo synthesized RNA can be detected with a simple procedure without the use of radiolabeling or antibodies. The kit principle relies on the incorporation of cell permeable 5-EU (Ethynyl uridine) into nascent RNA, but not into DNA, instead of its natural uridine analog. 5-EU can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo synthesized RNA in proliferating cells. Modified RNA is detected by click chemistry using an azide-containing dye that enables for multiplex analyses with other probes, or detection of RNA-interactive proteins for deeper biological insights. The kit provides sufficient materials for 100 assays for quantitative analysis by microplate reader (Ex/Em= 494/521 nm) and/or by fluorescence microscope. The kit includes Actinomycin D, an inhibitor RNA synthesis that serves as an experimental control.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• Conjugation
  red dye

• Gene target
  EZClick™   Global   RNA   Synthesis   Kit   FACS/Microscopy   Red  
• Short name
  EZClick™ Global RNA Synthesis Assay Kit (FACS/Microscopy), Red
• Technique
  Assay, FACS, assays, RNAs are to store at -70 C
• Host
  Assay
• Label
  Red
• Alternative name
  EZClick™ Global RNA Synthesis test reagent (flow cytometer cell sorting/Microscopy), Red Fluorescence
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Microscopy
• Concept
  Scope note: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
• Tree numbers
  • E01.370.350.515
  • E05.595
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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