EZClick™ EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence
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Catalog number
K946-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Flow Cytometry (Ex/Em 480/(530/590) nm) and Fluorescence Microscopy • Sample type- Adherent and suspension cells • Species reactivity- Mammalian • Applications- This assay provides a convenient and accurate procedure to measure de novo DNA synthesized in biological samples.
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Detection Method
Flow Cytometry (Ex/Em 480/(530/590) nm) and Fluorescence Microscopy
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Species Reactivity
Mammalian
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Applications
This assay provides a convenient and accurate procedure to measure de novo DNA synthesized in biological samples.
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Sample Type
Adherent and suspension cells
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Features Benefits
Simple, fast, does not require lengthy incubation times
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
All living cells undergo division cycle, a biological process crucial for proliferation and inheritance. Cell-division cycle is a series of events resulting in two daughter cells containing replicas of DNA from the original DNA molecule. DNA replication occurs in the S phase of the cell cycle and involves de novo synthesis of genomic DNA from its precursors. The ability of monitoring detailed characterization of cell cycle and DNA synthesis in proliferating cells is fundamental in basic, and applied immunologic and oncologic studies. Accurate determination of the effect of biologically active reagents on DNA synthesis and cell cycle is of great importance in anti-cancer drug discovery and basic biology. Biovision’s EZClick™ EdU DNA Synthesis Monitoring Kit utilizes a novel approach that relies on incorporation of 5-EdU (5-ethynyl-2’deoxyuridine) as nucleoside analog to thymidine into newly synthesized DNA directly in the cell culture. Incorporation of EdU into genomic DNA in S-phase is detected based on a click reaction between the alkyne moiety of EdU and fluorescent azide. Compared to historically used BrdU, click reaction is carried in mild conditions and flow cytometry/fluorescence microscopy can be used for assessment of proliferating cells in the population. Our kit provides sufficient materials for 100 assays based on the protocol below.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Conjugation
red dye
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
EZClick™ EdU Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red
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Technique
FACS, dna
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Label
Red
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Alternative name
EZClick™ EdU cellular expantion/Desoxyribonucleic acid Synthesis reagent (flow cytometer cell sorting/Microscopy), Red Fluorescence
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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