EZClick™ Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

• Catalog number
  K717-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Flow Cytometry (Ex/Em 488/(530/590) nm) and Fluorescence Microscopy (Ex/Em 440/490 and 540/580 nm) • Sample type- Adherent and suspension cells • Species reactivity- Mammalian • Applications- This assay provides a convenient and accurate procedure to measure de novo Protein synthesized in biological samples.
• Detection Method
  Flow Cytometry (Ex/Em 488/(530/590) nm) and Fluorescence Microscopy (Ex/Em 440/490 and 540/580 nm)
• Species Reactivity
  Eukaryotes
• Applications
  This assay provides a convenient and accurate procedure to measure de novo phopholipid synthesized in biological samples.
• Sample Type
  Adherent and suspension cells
• Features Benefits
  Simple, fast, does not require lengthy incubation times
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phospholipids are major component of the bilayers of all plasma membranes. A single phospholipid molecule consists of a phosphate group on one end, called the "head," and two side-by-side chains of fatty acids that make up the "tails”. The phosphate head groups can be modified with organic molecules such as Choline (Cho). Cho-containing phospholipids (Phosphatidylcholines; PC) are critical for structural membrane integrity, cellular metabolism and signaling either as individual molecules or precursors of secondary messengers. Changes in global synthesis of Cho-containing phospholipids are an essential parameter in analysis of cellular response to both, physiological and pathological conditions, environmental stress, or drug treatment. To date, phospholipids biochemistry, cell biology and metabolism remain obscure, due to limited methods for their direct cellular visualization. Biovision’s EZClick™ Global Phospholipid Synthesis Assay Kit offers a simple and robust method to label and visualize newly synthesized phospholipids in vivo. Based on the metabolic incorporation of the choline analogs directly into their structure, modified phospholipid molecules can be detected with high sensitivity and spatial resolution by click chemistry with azide-containing dyes. This kit enables analyses of global biosynthesis, subcellular localization and turnover of Cho-containing phospholipids in cells. Cells show strong incorporation of Cho analogs into all classes of phospholipids that can be assayed by fluorescence microscopy, or quantified by FACS. We provide sufficient materials for 100 assays
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• Conjugation
  red dye

• Gene target
  EZClick™   Global   Phospholipid   Synthesis   Kit   FACS/Microscopy   Red  
• Short name
  EZClick™ Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red
• Technique
  Assay, FACS, assays
• Host
  Assay
• Label
  Red
• Alternative name
  EZClick™ Global Phospholipid Synthesis test reagent (flow cytometer cell sorting/Microscopy), Red Fluorescence
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Microscopy
• Concept
  Scope note: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
• Tree numbers
  • E01.370.350.515
  • E05.595
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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