EZClick™ Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
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Catalog number
K497-100
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Price
Please ask
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Size
100 assays
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Description
Labeling and detection of myristoylated proteins
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Summary
EZClick™ Myristoylated Protein Assay Kit (FACS/Microscopy): A highly specific, simple and robust method for labeling and detection of myristoylated proteins. 100 assays.
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Detection Method
Fluorometric (Ex/Em = 440/490 nm)
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Species Reactivity
Mammalian
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Applications
Identification and localization of myristic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications, Detection and quantification of biosynthesis, dynamics and turnover of myristic acid modified proteins
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Sample Type
Suspension or adherent cell cultures
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Features Benefits
Simple procedure, Robust method for labeling and detection of myristoylated proteins, Highly specific
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Myristoylation is a co- and post-translational modification of proteins. Myristoylated proteins are involved in cellular trafficking, some cellular functions and oncogenesis. In this modification, Myristic acid, a saturated fatty acid is covalently bond to a protein through the action of N-myristoyl transferase (NMT). NMT transfers the myristate moiety from Myristoyl-CoA to a N-terminal glycine of a target protein. NMT is associated with carcinogenesis, the upregulation of its expression and activity is observed in colorectal cancer, gall-bladder cancer and brain tumors. BioVision offers EZClick™ Myristoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of myristoylated proteins. We use Alkynyl Myristic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation, followed by click reaction with and azide-containing dye. This system offers a powerful method for imaging localization, trafficking, and dynamics of myristoylated proteins, or detection by FACS for quantitative studies. Labeled myristoylated proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
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Test
Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Gene target
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Short name
EZClick™ Myristoylated Protein Assay Kit (FACS/Microscopy),
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Technique
Assay, FACS, assays
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Host
Assay
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Label
green
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Alternative name
EZClick™ Myristoylated Protein test reagent (flow cytometer cell sorting/Microscopy), Green Fluorescence
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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