EZClick™ Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
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Catalog number
K418-100
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Price
Please ask
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Size
100 assays
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Description
for labeling and detection of Stearoylated proteins
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Summary
BioVision offers EZClick™ Stearoylated Protein Assay Kit (Red Fluorescence) a highly specific, simple and robust method for labeling and detection of Stearoylated proteins.
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Detection Method
Fluorescence Microscope Imaging and FACS analysis
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Species Reactivity
Mammalian
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Applications
Detection and quantification of biosynthesis, dynamics and turnover of stearic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications, Identification and localization of stearic acid modified proteins
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Sample Type
Suspension or adherent cell cultures
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Features Benefits
Simple procedure, Highly specific, Robust method for labeling and detection of Stearoylated proteins
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Stearoylation is one of the numerous types of post-translational modification in proteins. Stearoylated proteins are involved in the regulation of cellular processes such as apoptosis, growth and metabolism. In this modification Stearic acid, a saturated fatty acid is covalently bound to a protein through the action of long-chain-fatty-acid-[acyl-carrier-protein] ligase. In the presence of ATP, ligase then transfers a Stearate moiety to the target protein forming a carbon-sulfur bond (C=S). Recent studies showed a possible correlation between mitochondrial dysfunction and some diseases including neurodegenerative disease, cancer and aging. Furthermore, it is known that stearoylated Transferrin Receptor (TfR1) inhibits JNK activation thereby reducing mitofusin ubiquitination and restoring mitochondrial function. Studying stearoylation of proteins can shed a light on disease etiology and prevention. BioVision offers EZClick™ Stearoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of Stearoylated proteins. The kit uses Alkynyl Stearic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Stearoylated proteins or detection by FACS for quantitative studies. Labeled stearoylated proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
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Test
Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Conjugation
red dye
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Gene target
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Short name
EZClick™ Stearoylated Protein Assay Kit (FACS/Microscopy), Red
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Technique
Assay, FACS, assays
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Host
Assay
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Label
Red
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Alternative name
EZClick™ Stearoylated Protein test reagent (flow cytometer cell sorting/Microscopy), Red Fluorescence
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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