EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

• Catalog number
  K416-100
• Price
  Please ask
• Size
  100 assays

• Description
  for labeling and detection of Palmitoylated proteins
• Summary
  BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins.
• Detection Method
  Fluorescence Microscope Imaging and FACS analysis
• Species Reactivity
  Mammalian
• Applications
  Identification and localization of palmitic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications, Detection and quantification of biosynthesis, dynamics and turnover of palmytoylated proteins
• Sample Type
  Suspension or adherent cell cultures
• Features Benefits
  Highly specific, Simple procedure, Robust method for labeling and detection of Palmitoylated proteins
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Palmitoylation occurs when fatty acids, such as Palmitic Acid are covalently attached to the side chains of cysteine (and less frequently to serine, threonine side chains) in proteins. This type of post-translational modification greatly affects cellular trafficking, compartmentalization, and membrane tethering. Palmityltransferase (PAT), the enzyme responsible for this modification, transfers a palmitate moiety from palmitoyl-CoA to the thiol group of cysteine in the target protein. Compared to myristoylation, palmitoylation is reversible and the reverse reaction is catalyzed by thioesterases. The Palmitoylation/Depalmitoylation cycle plays an important role when modified protein shuttles between cellular compartments. PAT mutations are associated with many neurological diseases and cancer progression. BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins. The kit uses a modified Palmitic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This post translational modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Palmitoylated proteins, or detection by FACS for quantitative studies. Labeled Palmitoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• Conjugation
  red dye

• Gene target
  EZClick™   Palmitoylated   Protein   Kit   FACS/Microscopy   Red  
• Short name
  EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red
• Technique
  Assay, FACS, assays
• Host
  Assay
• Label
  Red
• Alternative name
  EZClick™ Palmitoylated Protein test reagent (flow cytometer cell sorting/Microscopy), Red Fluorescence
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Microscopy
• Concept
  Scope note: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
• Tree numbers
  • E01.370.350.515
  • E05.595
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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