Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

• Background: TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.
• Synonyms: TIMP-1, CLG1, EPA, HCI
• CAS Number: 9007-83-4
• UniProt: P01033 (Human), Q95KL9 (Macaca)
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG1
• Clone: 2A5
• Conjugation: Unconjugated
• Type: Monoclonal Antibody
• Applications: WB, ELISA, IHC
• Field of Research: Cellular Structures
• Purification Method: Protein A/G Chromatography
• Assay Principle: Antibody can be used for Western blotting (1-2 µg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 µg/mL). Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Hoikkala, S., et al. (2006). Tissue MMP-2 and MMP-9 [corrected] are better prognostic factors than serum MMP-2/TIMP-2--complex or TIMP-1 [corrected] in stage [corrected] I-III lung carcinoma. Cancer Lett. 236(1):125-132 2. Bodden, M.K., et al. (1994). Functional domains of Human TIMP-1 (tissue inhibitor of metalloproteinases). J. Biol. Chem. 269(29):18943-18952
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB