Mouse anti Human myc

• Background:

  Human c-myc belongs to a family of 5 myc oncogenes. Amplification of c-myc has been found in several types of Human tumors including breast and colon carcinomas. Induction of c-myc in cells grown in the appropriate growth factors or co-expression with survival genes, such as bcl-2 causes cell proliferation. In the abscence of these factors, c-myc expression causes cell death. c-myc gene gives rise to at least two nuclear phosphoproteins of 64kDa and 67kDa.

• Synonyms:

  cmyc, Myc proto-oncogene Protein, transcription factor p64

• UniProt:

  P01106 (Human), P01108 (Mouse), P09416 (Rat)

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Isotype:

  IgG2a

• Clone:

  8

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Source:

  Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length Human c-Myc protein and mouse myeloma cells.

• Applications:

  WB, IP, IHC

• Field of Research:

  Signal Transduction

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  Applications: Western Blot, Immunoprecipitation (Both with and without SDS) and immunohistochemistry (see image)

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Alitalo K, Schwab M, Lin CC, Varmus HE, Bishop JM. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a Human colon carcinoma. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1707-11._x000D_ _x000D_ 2. Persson H, Hennighausen L, Taub R, DeGrado W, Leder P. Antibodies to Human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984 Aug 17;225(4663):687-93.

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4