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Mouse anti Human CD69

CAT: 0579-0691Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0579-0691Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Anti Human CD69 recognizes a very early activation antigen on T , B cells and macrophages. Also called AIM or activation inducer molecule, CD69 is a gp28/34 disulfide bonded homodimer with a Mr of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride. The CD69 molecule is also present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen. It is involved in lymphocyte signal transduction.
CAS Number
9007-83-4
Synonyms
CD69
UniProt
Q07108
Host
Mouse
Species Reactivity
Human
Isotype
IgG1
Clone
SMM
Conjugation
Unconjugated
Type
Single-Color Reagent
Applications
Flow Cytometry
Field of Research
Flow Cytometry
Purification Method
Protein A/G Chromatography
Assay Principle
PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
Shipping Conditions
Room Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
Flow Cytometry Staining

UniProtKB · Q07108

Early activation antigen CD69

CD69_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q07108
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD69
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Ligand

Lectin

Molecular function

Receptor

Domain

Signal-anchorTransmembraneTransmembrane helix

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