Mouse anti Human CD45

CAT: 0579-0451Size: 100 µgDry Ice: NoHazardous: No

1 / 1

CAT#:0579-0451Size:100 µg

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Background

Identification of all Human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M. W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow.

CAS Number

9007-83-4

Synonyms

CD45

UniProt

P08575

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Clone

BHPT-1

Conjugation

Unconjugated

Type

Single-Color Reagent

Applications

Flow Cytometry

Field of Research

Flow Cytometry

Purification Method

Protein A/G Chromatography

Assay Principle

PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 µL per test rather than 10 µL. Excites at 488nm and emits at 670nm. Store protected from light.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1 .Leukocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford University Press; 1989:628-634 2. Association of CD2 and CD45 on Human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May; 345(6270):71-4 Cy-5 + Portions of this product are manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Shipping Conditions

Room Temperature

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

Functional Analysis

Flow Cytometry Staining

UniProtKB · P08575

Receptor-type tyrosine-protein phosphatase C

PTPRC_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P08575
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: PTPRC
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 3.1.3.48
Processing: Precursor
Secondary accessions: A0A0A0MT22, A8K7W6, Q16614, Q9H0Y6, X6R433

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSynapse

Disease

Disease variantSCID

PTM

GlycoproteinPhosphoprotein

Biological process

Host-virus interaction

Molecular function

HydrolaseProtein phosphatase

Domain

RepeatSignalTransmembraneTransmembrane helix

Related Products

Catalog	Name
GM-4061-CE/IVD	Mouse anti CD45
GM-4061	Mouse anti CD45
GM-4062-CE/IVD	Mouse anti CD45, conjugated to FITC
GM-4062	Mouse anti CD45, conjugated to FITC
GCT-201-CE/IVD	COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)
GCT-201	COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)
0441	Mouse anti Human CD44
MUB3002P1	Mouse Anti Human CD4
0041	Mouse anti Human CD4
0043	Mouse anti Human CD4, conjugated with Biotin

Alternative Products

Catalog	Name
PT_74977-01	CD45, Mouse Anti Human (CD45 Antibody)
PT_74654-01	CD45, Mouse Anti-Human (CD45 Antibody)
MBS213183-01	MOUSE ANTI HUMAN CD45
31-3484-0.1	Mouse Anti-Human CD45