Mouse anti Human Rit

• Background:

  Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and Human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

• UniProt:

  Q92963

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse

• Isotype:

  IgG1

• Clone:

  14G7

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Source:

  Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length Human Rit protein and mouse myeloma cells.

• Applications:

  WB

• Field of Research:

  Signal Transduction

• Purification Method:

  Protein A or G purified

• Assay Principle:

  Antibody detects recombinant Rit protein expressed in 293 cells by Western blot analysis at a concentration of 0.5 to 1 µg/mL. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa)._x000D_Does not cross-react with Rin protein. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7,5 and 3 mM sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Rusyn, E.V., et al. Rit, a non-lipid-modified Ras-related protein, transforms NIH3T3 cells without activating the ERK, JNK, p38 MAPK or PI3K/Akt pathways. Oncogene 2000, 19, 4685-4694._x000D_ 2. Lee, C.H. et al. Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of ras proteins. J. Neurosci. 1996, 16, 6784-6794._x000D_ 3. Shao, H., et al. Biochemical characterization of the Ras-related GTPases Rit and Rin. Arch. Biochem. Biophys. 1999, 371, 207-219.

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4