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Mouse anti Human CD7

CAT: 0579-0071Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0579-0071Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Identification of Human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cells. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow.
CAS Number
9007-83-4
Synonyms
T-Cell Antigen CD7, GP40, T-Cell leukemia Antigen, TP41, Leu-9
UniProt
P09564 (Human)
Host
Mouse
Species Reactivity
Human
Isotype
IgG2a
Clone
MG34
Conjugation
Unconjugated
Type
Single-Color Reagent
Applications
Flow Cytometry
Field of Research
Flow Cytometry
Purification Method
Protein A/G Chromatography
Assay Principle
PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Isolation and characterization of the genomic Human CD7 gene: structural similarity with the murine Thy-1 gene. Schanberg, L.E., Fleener, D.E., Kurtzberg, J., Haynes, B.F., Kaufman, R.E.; Proc. Natl. Acad. Sci. USA , 1991 Jan 1;88(2):603-7. 2. Identification of novel B-lineage cells in Human fetal bone marrow that coexpress CD7. Grumayer, E.R., Griesinger, F., Hummell, D.S., Brunning, R.D., Kersey, J.H.; Blood, 1991 Jan ; 77(1):64-8. 3. Genuine CD7 expression in acute leukemic and lymphoblastic lymphoma. Osada, H., Emi, N., Ueda, R., Seto, M., Koike, K., Suchi, T., Kojima, S., Obata, Y., Takahashi, T.; Leuk. Res. 1990;14(10):869-77. 4. Inhibition of alloresponsive naive and memory T cells by CD7 and CD25 antibodies and by cyclosporine. Akbar, A.N., Amlot, P.L., Ivory,K., Timms, A., Janossy, G.; Transplantation, 1990 No;50(5):823-9. 5. Comparsion of outcome, clinical, laboratory, and immunological features in 164 children and adults with T-ALL. Garand, R., Vannier, J.P., Bene, M.C., Faure, G., Favre, M., Bernard, A,. Leukemia,1990 No;4(11):739-44.
Shipping Conditions
Room Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
Flow Cytometry Staining

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