Mouse anti Human LEF-1 alternate exon

• Background:

  Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody is specific for an epitope between a.a. 227-231. This sequence lies within the context dependent activation domain of LEF-1 and appears to be an alternate splice variant exon of the LEF-1 gene

• Synonyms:

  Mouse Anti Lef-1, Lymphoid Enhancer Factor 1

• UniProt:

  Q9UJU2

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  1C3

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Applications:

  WB

• Field of Research:

  Signal Transduction

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  Detect LEF-1 protein containing the alternately spliced exon between amino acids 212-241 by Western blot at 1-5 µg/mL. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Riese, J., et al., LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic. Cell 1997, 88, 777-787 2. Giese, K., et al. Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1. Proc. Natl. Acad. Sci. USA 1997, 94, 12845-12850 3. Behrens, J., et al. Functional interaction of beta-catenin with the transcription factor LEF-1. Nature 1996, 382, 638-642 4. Giese, K. and Grosschedl, R. LEF-1 contains an activation domain that stimulates transcription only in a specific context of factor-binding sites. EMBO J. 1993, 12, 4667-4676 5. Hovanes, K., et al., The Human LEF-1 gene contains a promoter preferentially active in lymphocytes and encodes multiple isoforms derived from alternative splicing. Nucleic Acids Res. 2000, 28, 1994-2003

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4